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. 2022 Mar 28;12(16):9704–9724. doi: 10.1039/d1ra09393b

Analytical parameters of conventional methods in detection of LPS.

Method Sample Technique LOD/sensitivity Ref.
Cultivation Culture & plating 1.5 CFU mL−1 50
LAL Milk Chromogenic assay ∼103 CFU mL−1 45
LAL Beef Violet red bile agar overlay 5067.6 ng g−1 51
LAL Physiological Violet red bile agar overlay 7.00–7.49/g−1 52
LAL Milk 1 : 104 to 1 : 109 48
LAL Plasma 0.005 to 0.001 μg mL−1 53
ELISA Complex mixtures ELISA using poly-l-lysine 1 μg mL−1 54
ELISA Murine Sandwich capture ELISA 1 ng mL−1 55
ELISA Clinical ELISA-bacteriophage receptor protein 0.05 EU mL−1 up to 500 EU mL−1 56
ELISA Human biofluid ELISA-high coating efficiency ∼0.2 μg 57
Kit Biological Endpoint chromogenic assay 0.1 EU mL−1 58
Kinetic-QCL Clinical Endpoint chromogenic assay 0.005 EU mL−1 59
Pyrosate kit Clinical Gel clot assay 1.0 EU mL−1 60
EndoLISA Biological Fluorescence 0.005 EU mL−1 56