Analytical parameters of conventional methods in detection of LPS.
| Method | Sample | Technique | LOD/sensitivity | Ref. |
|---|---|---|---|---|
| Cultivation | — | Culture & plating | 1.5 CFU mL−1 | 50 |
| LAL | Milk | Chromogenic assay | ∼103 CFU mL−1 | 45 |
| LAL | Beef | Violet red bile agar overlay | 5067.6 ng g−1 | 51 |
| LAL | Physiological | Violet red bile agar overlay | 7.00–7.49/g−1 | 52 |
| LAL | Milk | — | 1 : 104 to 1 : 109 | 48 |
| LAL | Plasma | — | 0.005 to 0.001 μg mL−1 | 53 |
| ELISA | Complex mixtures | ELISA using poly-l-lysine | 1 μg mL−1 | 54 |
| ELISA | Murine | Sandwich capture ELISA | 1 ng mL−1 | 55 |
| ELISA | Clinical | ELISA-bacteriophage receptor protein | 0.05 EU mL−1 up to 500 EU mL−1 | 56 |
| ELISA | Human biofluid | ELISA-high coating efficiency | ∼0.2 μg | 57 |
| Kit | Biological | Endpoint chromogenic assay | 0.1 EU mL−1 | 58 |
| Kinetic-QCL | Clinical | Endpoint chromogenic assay | 0.005 EU mL−1 | 59 |
| Pyrosate kit | Clinical | Gel clot assay | 1.0 EU mL−1 | 60 |
| EndoLISA | Biological | Fluorescence | 0.005 EU mL−1 | 56 |