Comparison advantages and limitations of traditional methods in the detection of LPS.

Method	Advantages	Limitation	Ref.
Cultivation	It evaluates living (cultureable) microorganisms, recognizes live cells in the sample, easily quantifies the cells in the sample, and achieves high sensitivity in the appropriate medium	Advanced skills are required for optimal results, unique dietary supplements are required, and unique nutritional media are required for optimal growth. Time and resource intensive, relies on phenotypic biochemical characterization	61 and 62
LAL	Acceptable sensitivity & specificity, cost effective	Time consuming, complicated procedures, serious ecological problems	63 and 64
ELISA	Rapid, cost saving, acceptable sensitivity, and specificity, flexibility	Difficult antibody pretreatment, cross reactive between the captured antibody and target, need for standard ELISA kit	65 and 66