Table 1.
Primer sequences used for qPCR.
| Genes | 5' to 3' sequence | Tm (°C) | Size /bp |
|---|---|---|---|
| MyoD F | TCTATGATGACCCGTGTTTCG | 58.3 | 124 |
| MyoD R | TGCACCGCAGTAGGGAAGT | 58.7 | |
| MyoG F | GGGCTATGAGCGGACTGA | 56.3 | 230 |
| MyoG R | GCAGGGTGCTCCTCTTCA | 56.1 | |
| IGF-1R F | GCCAAACTCAACCGTCTA | 51 | 237 |
| IGF-1R R | ATTGCCCAACCTGCTGT | 53.5 | |
| β-actin F | CTGTGCCCATCTACGAGGGCTAT | 64.5 | 155 |
| β-actin R | TTTGATGTCACGCACGATTTCC | 63.2 | |
| mmu-miR-133a-3p F | TTTGGTCCCCTTCAACCAGCTG | ||
| U6 F | GCTTCGGCAGCACATATACT | 55.6 | 134 |
| U6 R | GTGCAGGGTCCGAGGTATTC | 59.2 |
mmu-miR-133a-3p, reverse primer Gene_ID,MIMAT0000145.