Table 3.
Skeletal muscle tissue and serum antioxidant capacity in mice.
| Groups | MDA | T-AOC | SOD | CAT | GSH-PX | |||||
|---|---|---|---|---|---|---|---|---|---|---|
|
Serum (nmol/mL) |
Skeletal muscle (nmol/mg protein) |
Serum (μmol/mL) |
Skeletal muscle (μmol/mg protein) |
Serum (U/mL) |
Skeletal muscle (U/mg protein) |
Serum (U/mL) |
Skeletal muscle (U/mg protein) |
Serum (U/mL) |
Skeletal muscle (U/mg protein) |
|
| C | 5.60 ± 0.38 | 3.26 ± 0.32 | 1.99 ± 0.11 | 0.65 ± 0.05 | 59.58 ± 2.29 | 32.42 ± 1.68 | 22.67 ± 0.76 | 12.83 ± 0.48 | 190.79 ± 4.99 | 26.91 ± 0.63 |
| M | 13.88 ± 0.63 | 8.14 ± 0.34 | 1.19 ± 0.09 | 0.32 ± 0.02 | 30.34 ± 1.48 | 17.88 ± 1.12 | 10.81 ± 0.63 | 5.36 ± 0.31 | 95.06 ± 8.03 | 11.36 ± 0.81 |
| VC | 8.37 ± 0.36 | 5.21 ± 0.37 | 1.53 ± 0.11 | 0.45 ± 0.02 | 42.89 ± 1.80 | 24.70 ± 1.51 | 14.86 ± 0.56 | 8.79 ± 0.70 | 127.75 ± 6.18 | 18.06 ± 0.79 |
| LCBP | 7.27 ± 0.42** | 4.28 ± 0.39** | 1.74 ± 0.07** | 0.55 ± 0.03** | 47.42 ± 1.94** | 27.68 ± 1.34** | 16.97 ± 0.71** | 10.30 ± 0.64** | 152.36 ± 6.58** | 21.73 ± 0.87** |
| L-M | 17.94 ± 0.36 | 10.42 ± 0.45 | 0.80 ± 0.06 | 0.19 ± 0.01 | 18.92 ± 1.28 | 11.39 ± 0.78 | 6.76 ± 0.42 | 3.22 ± 0.23 | 66.40 ± 5.75 | 7.59 ± 0.74 |
| L-VC | 11.82 ± 0.44 | 6.51 ± 0.35 | 1.25 ± 0.08 | 0.35 ± 0.02 | 33.85 ± 1.54 | 19.48 ± 1.12 | 12.42 ± 0.56 | 6.65 ± 0.34 | 114.61 ± 5.19 | 14.82 ± 1.35 |
| L-LCBP | 9.00 ± 0.398## | 5.02 ± 0.22## | 1.42 ± 0.05## | 0.42 ± 0.03## | 41.49 ± 2.20## | 22.63 ± 0.78## | 14.50 ± 0.47## | 8.67 ± 0.59## | 132.13 ± 4.99## | 18.69 ± 0.72## |
Values are expressed as mean ± standard deviation. M vs. LCBP,
p < 0.01; L-M vs. L-LCBP,
p < 0.01; LCBP, Lonicera Caerulea berry polyphenols; VC, vitamin C. Individuals in C, the control group, were fed for 6 weeks without exercise and were provided with the same amount of normal saline intragastrically at the same time as other groups at 22 ± 1 C; M and L-M. The normal temperature exercise model group and the low-temperature exercise model group were subjected to exercise at 25 C and −5°C for 6 weeks, respectively, 6 days a week, for 45 min/d, on a platform with a slope of 0. The first week included subjection to adaptive training, in which the initial speed of 11 m/min was increased at a rate of 1 m/min every day. From second week onward, the speed was increased by 1 m/min every week, and the same amount of normal saline was administered 1 h before exercise. VC and L-VC mice in the positive groups were subjected to exercise in the same way as the model group for 6 weeks, and they received VC intragastric administration (60 mg/kg) at 1 h before subjection to exercise at 25 °C and −5°C. LCBP and L-LCBP (250 mg/kg) were administered intragastrically 1 h before subjection to exercise at 25 and −5°C.