Table 1:

List of injection sites, with a brief description of the procedure and comparison of each method

Site	Description	Strengths	Limitations	References
AC	Implantation of tumor cells through a corneal defect into the AC	Reliable formation of iris melanomas in an immune-privileged space	Metastases limited and cells can seed extraocular space	In hamsters (16–20) In mice (21–32) In rabbits (33,34–39)
Suprachoroid	Transcorneal or transconjunctival delivery of cells directly above the choroid	Reliable formation of choroidal melanoma with metastases. Transcorneal approach has minimal extraocular tumor growth.	Deep corneal stromal perforation in transcorneal approach and seeding of cells into the subconjunctival space in transconjunctival approach.	In mice (23,29,40–51) In rabbits (52–60) In rats (61,62)
Subchoroid	Placement of cells inside an iatrogenic choroidal detachment after retinotomy	Reliable formation of choroidal melanomas within a short period of time	Complex technique with retinotomy. Complications such as vitreous hemorrhage, vitreous cell seeding, retinal detachment, and proliferative vitreoretinopathy.	In hamsters (63) In rabbits (37,64–69)
Intravitreal	Injection of tumor cells directly into the vitreous through the sclera	Effective primary tumor growth in the choroid	No extrapulmonary metastases. Changes in the tumor environment. Possible seeding into the anterior chamber.	In mice (70–73)

AC, anterior chamber.