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. 2022 Mar 9;13:841692. doi: 10.3389/fimmu.2022.841692

Table 1.

In vivo studies showing the anti-inflammatory action of Ang-(1–7) in the brain.

Reference Pathology Species Treatment Neuroinflammation Functional outcome Other findings
Hoyer‐Kimura et al. (124) Cognitive impairment mouse glycosylated Ang-(1–7) (PNA5), 50–500 μg/kg subcutaneously injected for 24 d ↓ pro-inflammatory cytokine (TNF-α) ↑cytokines (IL-1α, IL-2, IL-5, IL-13, IL-17, IL-10) ↑ memory ↓ NfL (both with Ang-(1–7) and PNA5)
Rabie et al. (125) Parkinson’s
disease
rat Ang-(1–7), 240 pg daily injected into the striatum for 1 w ↓ pro-inflammatory markers (RAGE and HMGB-1, NF-κB, p65 TNF-α, PARP-1) ↑ motor performance rescue of dopaminergic neurons
Arroja et al. (126) Stroke rat Ang-(1–7), 1 nmol/h intracerebroventricular infusion with osmotic pump for 6 w no effect ↓ tissue damage
↑ BBB damage
↑Nox1
Cao et al. (127) Alzheimer’s disease mouse Ang-(1–7), 400 ng/kg/min, with osmotic minipump for 4 w ↓ microgliosis (CD68, IBA1) ↑ memory ↓ amyloid deposits
↑ neuronal count
Hay et al. (128) Cognitive impairment rat glycosylated Ang-(1–7) (PNA5), 1.0 mg/kg subcutaneously for 21 d ↓ microglial activation (IBA1)
↓ pro-inflammatory cytokines (TNF-α, IL-7) ↑anti-inflammatory cytokine (IL 10)
↑ memory PNA5:
↑ blood-brain permeability
↑ stability/bioavailability
↓ ROS
Janatpour et al. (129) TBI mouse Ang-(1–7), 1 mg/kg s.c. by osmotic pumps 1 or 6 h post-injury, until 3 or 29 d. ↓ astrogliosis (GFAP)
↓ microgliosis (IBA1)
↑ learning and memory
↑ motor
↓ lesion volume
↓ neuronal death
↓ vessel density
Regenhardt et al. (66) Stroke rat Ang-(1–7), 100 pg intracerebroventricular infusion with osmotic pump for 6 w ↓ microgliosis (IBA1)
↓ pro-inflammatory cytokines (IL-1α, IL-6)
↑ lethargy ↑ survival
↓ brain hemorrhages
Regenhardt et al. (120) Stroke rat Ang-(1–7), 1.1 nM; 0.5 μl/h in the brain by osmotic pumps ↓ microgliosis (IBA1)
Rabie et al. (130) Parkinson’s disease rat Ang-(1–7), 240 pg daily injected into the striatum for 1 w ↓ pro-inflammatory markers (p-MAPK p38/NF-κB p65) ↑ motor performance
Hay et al. (131) Heart failure mouse Ang-(1–7), 500 pg/kg/h s.c. by osmotic pump for 4 w ↑ neuroprotection markers (CXCL12, CXCL13,G-CSF,CCL2,IL-16,IP-10,sICAM and IL-1ra) ↑ memory
Goldstein et al. (132) Brain damage by Shiga toxin rat Ang-(1–7), 200 pg daily injected into the hypothalamic area for 8 d ↓ microglial cell number
↓ astrocytic damage
↓ neuronal damage
↓ demyelination
Bihl et al. (133) Stroke mouse Ang-(1–7) (240 pg/kg/h) minipump infusion ↓ pro-inflammatory markers (TNF-α, MCP-1, IL-8, NF-κB) ↓ sensorimotor deficits ↑ vascular remodeling
↓ hemorrhage volume