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. 2022 Mar 9;13:811551. doi: 10.3389/fimmu.2022.811551

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Copyright © 2022 Wu, Roberto, Knupp, Greninger, Truong, Hollingshead, Kenerson, Tuefferd, Chen, Koelle, Horton, Jerome, Polyak, Yeung and Crispe

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Delta-delta Ct analysis showing up-regulated genes post- poly-I:C or LPS stimulation in chronic HCV-infected and DAA-cured liver slices. The relative gene abundance was normalized to to the arithmetic mean of Ct values of ACTB, GAPDH and HPRT1. The relative abundance was further normalized to the day 7 time zero measurements. Statistical significance was based on two-tailed Mann-Whitney test. *, statistical significantly different between non-infected versus chronic HCV liver slices. †, statistical significantly different between non-infected versus DAA-cured liver slices. ‡, statistical significantly different between chronic HCV versus DAA-cured liver slices. Levels of statistical significance are indicated thus: *P < 0.05 **P < 0.01. The mean and standard deviation within each group are plotted.