Table 3.
Product quality data for MERS-7.7G6 at 5, 50 and 200 L scales
| Batch | HPSEC % Monomer |
cIEF main peak pI | cIEF % main peak |
cIEF % acidic peaks |
cIEF % basic peaks |
Reduced CGE % purity |
Non-reduced CGE % purity |
Intact mass (Da) |
|---|---|---|---|---|---|---|---|---|
| 5 L D8 | 100.0 | 8.8 | 65.2 | 22.0 | 12.9 | 98.7 | 96.6 | 149800 |
| 5 L D12 | 99.3 | 8.8 | 62.4 | 29.0 | 8.6 | 98.3 | 95.0 | 149798 |
| 50 L D8 | 99.1 | 8.7 | 62.3 | 27.9 | 9.7 | 98.4 | 95.4 | 149804 |
| 50 L D12 | 99.0 | 8.7 | 59.0 | 32.1 | 8.9 | 97.6 | 94.7 | 149804 |
| 200 L D12 | 99.3 | 8.7 | 68.3 | 22.2 | 9.5 | 95.5 | 98.8 | 149801 |
| % RSD | 0.4 | 0.6 | 5.5 | 16.6 | 17.4 | 1.3 | 1.7 | 0.0 |
Purified antibody from day 8 (D8) or day 12 (D12) harvests was analyzed by HP-SEC, cIEF, CGE, and LC/MS.