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. 2022 Mar 9;9:840689. doi: 10.3389/fcvm.2022.840689

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Copyright © 2022 Chevalier, Selim, Castro, Cuvilly, Baste, Richard, Pareige and Bellien.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tomography 3D FIB-SEM under physiological conditions: (A) Micrographs extracted from FIB-SEM stack at different z-position. Stack images were acquired from 37 “slice and view” images with a step of 10 nm. Glycocalyx (G) (yellow arrows) appears as white dense-packed material at the endothelial (En). White arrows point endothelial vesicles filled with glycocalyx. (B) Volume reconstruction: Glycocalyx is thresholded as green part while the endothelium is in red. Note the presence of glycocalyx inside the intracytoplasmic vesicles. Scale bar = 1 μm.