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. 2022 Mar 9;9:840689. doi: 10.3389/fcvm.2022.840689

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Copyright © 2022 Chevalier, Selim, Castro, Cuvilly, Baste, Richard, Pareige and Bellien.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology of the glycocalyx under IR-CPB conditions imaged by EM combined techniques. (A,B) Overview micrographs acquired by SEM observations on resin embedded ultrathin sections (scale bar: 1 μm). (C–F) Higher resolution TEM images showing the glycocalyx weakly developed at the endothelium, red blood and platelet surface (scale bar = 100 nm). (F) Presence of large components (white arrows) in luminal space reacting with lanthanum salt (yellow arrows) as shown by EFTEM imaging (G,H) scale bar = 0.5 μm. Ar, Artery; BL, Basal Lamina; En, Endothelium; Lu, Lumen; P, Platelet; RBC, Red Blood Cell.