TABLE 1.
Bacterial strains
| Strain | Relevant genotypea | Source or reference |
|---|---|---|
| DM4100 | Wild type | 20 |
| KD66 | DM4100 gyrA (Nalr S83L) | 17 |
| KD1366 | gyrA (Nalr S83L) parC (Cipr S80L)b | 25 |
| KD1373 | DM4100 parC (Cipr S80L) | 25 |
| KD1525 | gyrA (Nalr D82A) parC (Cipr S80L) | This work, as described in reference 25 |
| KD1721 | DM4100 gyrA (Nalr A51V) | This work, by P1-mediated transduction from T/r (6) |
| KD1911 | DM4100 gyrA (Nalr A67S) | This work, by P1-mediated transduction from P-10 (22) |
| KD1915 | DM4100 gyrA (Nalr G81C) | This work, by P1-mediated transduction from N-97 (22) |
| KD1909 | DM4100 gyrA (Nalr S83W) | This work, by P1-mediated transduction from P-18 (22) |
| KD1913 | DM4100 gyrA (Nalr D87N) | This work, by P1-mediated transduction from N-113 (22) |
| KD1917 | DM4100 gyrA (Nalr Q106H) | This work, by P1-mediated transduction from N-89 (22) |
| KD1957 | gyrA (Nalr A51V) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1721 zeg::Tn10c into KD1373 |
| KD1959 | gyrA (Nalr A67S) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1911 zeg::Tn10 into KD1373 |
| KD1961 | gyrA (Nalr G81C) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1915 zeg::Tn10 into KD1373 |
| KD1963 | gyrA (Nalr S83W) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1909 zeg::Tn10 into KD1373 |
| KD1965 | gyrA (Nalr A84P) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1975 zeg::Tn10 into KD1373 |
| KD1967 | gyrA (Nalr D87Y) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1977 zeg::Tn10 into KD1373 |
| KD1969 | gyrA (Nalr Q106H) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1917 zeg::Tn10 into KD1373 |
| KD1971 | gyrA (Nalr D87N) parC (Cipr S80L) | This work, by P1-mediated transduction from KD1913 zeg::Tn10 into KD1373 |
| KD1973 | DM4100 gyrA (Nalr D82A) | This work, by P1-mediated transduction from KD1525 into DM4100 (25) |
| KD1975 | DM4100 gyrA (Nalr A84P) | This work, by P1-mediated transduction from P-5 (22) |
| KD1977 | DM4100 gyrA (Nalr D87Y) | This work, by P1-mediated transduction from KD1677d (M. Friedman) |
a
Amino acid abbreviations: A, alanine; D, aspartic acid; C, cysteine; H, histidine; G, glycine; L, leucine; N, asparagine; P, proline; Q, glutamine; S, serine; V, valine; W, tryptophan; Y, tyrosine. The first letter represents the wild-type amino acid at the position indicated by the number. The second letter indicates the identity of the amino acid which was substituted for the original at that position.
b
By itself this parC allele confers no resistance because topoisomerase IV is a secondary target.
c
zeg::Tn10 encodes a tetracycline resistance gene that is 50% cotransducible with gyrA.
d
Obtained by selection on agar containing nalidixic acid (20 μg/ml).