Author response image 2. Postnatal L6 ablation does not affect L4 cell number at P7.

(A-C) Coronal sections of P7 cortices electroporated with DTR and DsRed at E11.5, when layer 6 neurons are produced. Without DT administration, DsRedpositive cells are mainly located in Tbr1-positive layer 6 at P7 (A). Upon DT administration at P0, the number of Tbr1-positive layer 6 cells was markedly reduced on electroporated (e.p.) side compared with control side at P7 (B). The number of RORC-immunoreactive layer 4 cells was not different between the hemispheres (C). (D) Quantification of the results. The number of RORC- and Tbr1-immunoreactive cells is presented as a percentage of control (mean ± SEM): Tbr1, 41.40 ± 6.44%; RORC, 104.25 ± 5.25%, N = 3 for each. L4, layer 4; L6, layer 6. Scale bars, 100 µm.