| Gene (M. musculus) |
Nrn1
|
GenBank |
BC035531 |
|
| Gene (M. musculus) |
Vgf
|
GenBank |
BC085134 |
|
| Gene (Homo-sapiens) |
HBEGF
|
GenBank |
BC033097 |
|
| Strain, strain background (Escherichia coli) |
DH5α |
Our lab |
|
Calcium chloride-treated competent cells |
| Strain, strain background (M. musculus) |
ICR |
SLC Japan |
Slc:ICR |
|
| Strain, strain background (M. musculus) |
C57BL/6J |
SLC Japan |
C57BL/6JJmsSlc |
|
| Genetic reagent (M. musculus) |
C57BL/6J-Tg(Slc6a4-cre)208Ito(5HTT-Cre, Sert-Cre, SLC6A4-Cre) |
RIKEN BRC Arakawa et al., 2014
|
RBRC10598C57BL/6J-Tg(Slc6a4-cre)208Ito
|
|
| Genetic reagent (M. musculus) |
ROSA26iDTR(B6-iDTR) |
The Jackson Laboratory Buch et al., 2005
|
Strain #007900C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J |
|
| Genetic reagent (M. musculus) |
R26R-EYFP |
The Jackson LaboratorySrinivas et al., 2001
|
Strain #006148B6.129 × 1-Gt(ROSA)26Sortm1(EYFP)Cos/J |
|
| Genetic reagent (M. musculus) |
Nrn1-KO |
This paper |
|
CRISPR-mediated Nrn1 null mice available upon request |
| Genetic reagent (M. musculus) |
Vgf-KO |
This paper |
|
CRISPR-mediated Vgf null mice available upon request |
| Antibody |
anti-RORC (Mouse monoclonal) |
Perseus Proteomics |
Cat#: PP-H3925-00 |
IF (1:800) |
| Antibody |
anti-GFP (Rabbit polyclonal) |
Invitrogen |
Cat#: A6455 |
IF (1:800) |
| Antibody |
anti-Iba1 (Rabbit polyclonal) |
Wako |
Cat#: 019-19741 |
IF (1:500) |
| Antibody |
anti-Brn2 (Goat polyclonal) |
Santa Cruz |
Cat#: sc-6029 |
IF (1:50) |
| Antibody |
anti-Ctip2 (Rat polyclonal) |
Abcam |
Cat#: ab18465 |
IF (1:200) |
| Antibody |
anti-Tbr1 (Rabbit polyclonal) |
Abcam |
Cat#: ab31940 |
IF (1:500) |
| Antibody |
anti-NeuN (Mouse monoclonal) |
Chemicon |
Cat#: MAB377 |
IF (1:400) |
| Antibody |
anti- Cux1 (Rabbit polyclonal) |
Santa Cruz |
Cat#: sc-13024 |
IF (1:100) |
| Antibody |
anti-RFP (Rabbit polyclonal) |
MBL |
Cat#: PM005 |
IF (1:1000) |
| Antibody |
anti-FLAG (Mouse monoclonal) |
Sigma-Aldrich |
Cat#: F1804 |
IF (1:1000) |
| Antibody |
anti-ssDNA (Rabbit polyclonal) |
MBL |
Cat#: 18,731 |
IF (1:300) |
| Antibody |
anti- RORβ (Rabbit polyclonal) |
Diagenode |
Cat#: pAb-RORβHS-100 |
IF (1:5000) |
| Antibody |
anti- NRN1 (Rabbit polyclonal) |
Santa Cruz |
Cat#: sc-13 25,261 |
IF (1:50) |
| Antibody |
anti-VGF (Goat polyclonal) |
Santa Cruz |
Cat#: sc-10381 |
IF (1:50) |
| Antibody |
anti-DIG (Sheep polyclonal) |
Roche |
Cat#: 11093274910 |
ISH (1:1000) |
| Recombinant DNA reagent |
pFLCI-Btbd3 (plasmid) |
Matsui et al., 2011
|
|
|
| Recombinant DNA reagent |
pCAG-DTR (plasmid) |
This paper |
|
HBEGF expression plasmid driven by CAG promoter |
| Recombinant DNA reagent |
pCAG-DsRed(plasmid) |
Zhao et al., 2011
|
|
|
| Sequence-based reagent |
Nrn1 sgRNA |
This paper |
Single-guide RNA targeting Nrn1
|
AGCATGGCCAACTACCCGCA |
| Sequence-based reagent |
Vgf sgRNA #1 |
This paper |
Single-guide RNA targeting Vgf
|
TCACGTTGCCGGCATCCGTC |
| Sequence-based reagent |
Vgf sgRNA #2 |
This paper |
Single-guide RNA targeting Vgf |
CGGTACTGTTGCAGGCACTGGACCGT |
| Sequence-based reagent |
Nrn1_FW
|
This paper |
PCR primer |
ACCAGGGAACTGAGCCTGAG |
| Sequence-based reagent |
Nrn1_RV
|
This paper |
PCR primer |
GGACTCACCTCCCTGCTATC |
| Sequence-based reagent |
Vgf-FW
|
This paper |
PCR primer |
GGTACCCAGAAGGAGGATTG |
| Sequence-based reagent |
Vgf-RV
|
This paper |
PCR primer |
TTGCTCGGACTGAAATCTCG |
| Sequence-based reagent |
Vgf-Seq
|
This paper |
Sequencing primer |
CTCAGCTCTGAGCATAATGG |
| Peptide, recombinant protein |
Diphtheria toxin |
Calbiochem |
Cat#: 322,326 |
|
| Commercial assay or kit |
HistoVT One |
Nacalai Tesque |
Cat#: 06380-05 |
|
| Commercial assay or kit |
M.O.M. Basic Kit |
Vector |
Cat#: BMK-2202 |
|
| Commercial assay or kit |
VENTASTAIN ABC Kit |
Vector |
Cat#: PK4000 |
|
| Commercial assay or kit |
Click-iT EdU Imaging Kit |
Thermo Fisher Scientific |
Cat#: C10337
|
|
| Commercial assay or kit |
DIG-RNA Synthesis Kit |
Roche |
Cat#: 11175025910 |
|
| Commercial assay or kit |
Plasmid Maxi Kit, Roche |
Genopure |
Cat#: 03143422001 |
|
| Chemical compound, drug |
NBT/BCIP |
Roche |
Cat#: 11681451001 |
ISH (1:50) |
| Chemical compound, drug |
DiA |
Thermo Fisher Scientific |
Cat#: D3883 |
|
| Chemical compound, drug |
DiI |
Thermo Fisher Scientific |
Cat#: D3911 |
|
| Software, algorithm |
Metamorph software |
Molecular Devices |
|
|
| Software, algorithm |
ImageJ |
https://imagej.nih.gov/ij/
|
ImageJ v1.52 |
|
| Software, algorithm |
GraphPad Prism |
GraphPad Software |
GraphPad Prism 5.0 |
|
| Other |
DAPI stain |
Wako |
Cat#: 340-07971 |
1:1000 |
