Table 9.
Assessment of hematopoietic supporting activity.
| Author | Results | Method of assessment |
|---|---|---|
| Bacigalupo et al. (47) | • No difference | • MSC long term co-culture with BMNC (LTC-IC) |
| Bueno et al. (37) | • No difference | • MSC co-cultures with UCB-CD34+ cells; proliferation rate, apoptosis frequency, cell cycle analysis and CFU-assay |
| Chao et al. (30) | • Less proliferation of PBMCs in AA-MSCs co-cultures, however no difference when cultured only with AA-MSC CM |
• SC co-culture with PBMC; PBMC proliferation |
| Chaturvedi et al. (31) | • RT-qPCR (after LPS stimulation): – Higher expression of TNF-α, G-CSF and SDF-1α expression in AA-MSCs – Lower MIP-1α expression in AA-MSCs – No difference in SCF and TGF-β expression • Measurement in culture supernatant: similar as RT-qPCR, however also no difference in SDF-1α |
• RT-qPCR and ELISA; MIP-1α, TNF-α, G-CSF, SDF-1α, SCF, and TGF- expression in LPS induced MSCs |
| Hamzic et al. (36) | • Worse ability to form adherent stromal layers by AA-MSCs at 5 and 6 weeks • Worse colony-forming and proliferation potential of normal CD34+ cells in AA-MSC cross cultures at 6 weeks |
• Adherent stromal layer formation • MSC cross-cultures between BM-CD34+ cells (AA/normal) and MSCs (AA/normal; hematopoietic colony formation, non-adherent cell proliferation |
| Jiang et al. (39) | • Lower FGF-2 gene expression in AA-MSCs • Lower FGF-2 levels in BM of AA-patients |
• RT-qPCR and ELISA; FGF-2 expression and levels in BM plasma |
| Lu, S et al. (32) | • Lower CD106 and NF-κB expression in AA-MSCs • Lower vasculogenesis ability AA-MSCs • Lower VEGF levels in CM of AA-MSCs • Lower levels of CD34+, CD41+ and CD61+ cells in AA- MSC co-cultures • Less CFU-GM and CFU-mixed cell colonies in AA-MSC co- cultures • Less and smaller CFU-MK colonies in AA-MSC co-cultures |
• Illumina sequencing, RT-qPCR, western blot analysis, NanoPro analysis; CD106 and NF-κB expression • Matrigel plug assay; in vitro capillary tube-like formation • ELISA; VEGF production by MSCs • MSC co-culture with UCB-CD34+ cells; CFU and CFU-MK assay |
| Shipounova et al. (45) | • Lower expression of ANG-1 and VCAM-1 & higher expression of VEGF after 3 weeks • Normalization of gene expression after 6 weeks |
• RT-qPCR; ANG-1, VCAM-1, VEGF in adherent cells of long-term bone marrow cultures |
| Michelozzi et al. (33) | • No difference | • RT-qPCR; TGFB1, IL-6, DDK1 |