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Lipopolysaccharide-EK (LPS-EK) induces inflammation in macrophages. RAW cells were incubated with LPS-EK for 6 h. Gene expression of (A) IL-6, (B) IL-1β, (C) TNF-α, (D) MIP-2, and (E) NLRP3 was quantified by quantitative real-time PCR and normalized to that of the housekeeping gene ß-actin. Data are expressed as the mean ± SEM of four independent experiments performed in triplicate (n = 4). *p < 0.05 vs. CT, Mann-Whitney test. LPS 0.1: 0.1 µg/ml LPS-EK, LPS 1: 1 µg/ml LPS-EK.
