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. 2022 Mar 9;10:823450. doi: 10.3389/fcell.2022.823450

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Copyright © 2022 Yaker, Tebani, Lesueur, Dias, Jung, Bekri, Guerrera, Kamel, Ausseil and Boullier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EV-LPS increase antioxidant marker gene expression in smooth muscle cells. MOVAS-1 cells were incubated with EVs-derived RAW cells for 24 h. Gene expression of (A) SOD-1, (B) SOD-2, (C) Nrf2, and (D) Keap1 was then quantified by quantitative real-time PCR and normalized to that of the housekeeping gene ß-actin. Data are expressed as the mean ± SEM of four independent experiments performed in triplicate (n = 4). *p < 0.05 vs. CT, $p < 0.05 vs. EV-CT, Mann-Whitney test. EV-CT: EV-derived from untreated macrophages, EV-LPS: EV-derived from LPS-EK-treated macrophages.