TABLE 6.

Heterologous expression of proteins and nematicidal activity of recombinant E. coli cells.

Protein	Locus tag	Function	Methodology	Killinga
Proteases
	VT47_24200	Hemolysins and related proteins containing CBS domains R	Genomics	±
Metalloprotease	VT47_14210	ZnMc superfamily	Genomics	+
	VT47_14120	Protease M4 superfamily	VirlentPred	+ +
	VT47_04845	Peptidase superfamily M48	VirlentPred	+
	VT47_20845	Putative metalloprotease Psyr	VirlentPred	+
	VT47_20605	Zn_peptidase superfamily	VirlentPred	+
Alkaline protease	VT47_14725	Alkaline protease secretion protein AprE	VirlentPred	+
	VT47_14720	Alkaline protease secretion protein AprF	VirlentPred	+ +
Serine protease	VT47_18880	Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain	Genomics	±
	VT47_05790	Easterase_lipase super family	VirlentPred	+
	VT47_21025	Easterase_lipase super family	VirlentPred	+ +
Esterase	VT47_17505	NLPC_P60 family	VirlentPred	+ +
	VT47_09750	Methyl-accepting chemotaxis protein	VirlentPred	+ +
Chitinase	VT47_10710	Methyl-accepting chemotaxis protein	VirlentPred	+ +
MCP	VT47_04285	Methyl-accepting chemotaxis protein	VirlentPred	+ +
	VT47_03775	Methyl-accepting chemotaxis protein	VirlentPred	+ +
	VT47_13370	Methyl-accepting chemotaxis protein	VirlentPred	+ +
	VT47_05595	Chemotaxis family	VirlentPred	+
	VT47_06255	RtxA structural toxin protein	VirlentPred	+ +
CP	VT47_04590	RTX toxins	VirlentPred	+
RTX	VT47_14740	RTX toxins and related Ca2+-binding proteins	Genomics	+ +
	VT47_21930	Iron-sulfur cluster assembly protein	Transcriptomics	+ +
	VT47_01265	Conserved blocks	Transcriptomics	+
Bacterioferritin	VT47_17830	P-loop-NTPase superfamily	VirlentPred	+ +
IscA	VT47_06970	TolA colicin import membrane protein	VirlentPred	+ +
Undefined protein	VT47_16070	Pertactin-like passenger domains	VirlentPred	+ +
Antimicrobial peptide	VT47_19645	PBP1-YraM-Lppc-lipoprotein like	VirlentPred	+
Adhesin
Lipoprotein

^aAssay was performed in triplicate and mean values are shown. The results of killing were grouped as +++ ≥ 80%, ++= 50–80%, += 20–50% and ±= 10–20%.