TABLE 4.
Kirby-Bauer disk diffusion assay for selected quinolones
| Strain | Relevant genotype | Zone diameter (mm)a
|
||||
|---|---|---|---|---|---|---|
| Nor | Oflox | Cipro | Lom | Nal | ||
| PAO1 | Wild type | 28 | 20 | 31 | 23 | 7 |
| 2547 | PAO1 mutant | 20 | 12 | 27 | 13 | 7 |
| 2548 | 2547 mutant | 19 | 10 | 26 | 11 | 7 |
| 3580 | 2547Δ(amrB) | 28 | 18 | 36 | 24 | 7 |
a
Disk diameter is 6 mm. Disk content (in μg/ml) and resistance and sensitivity ranges (R and S, respectively; also in μg/ml) for the drugs tested were as follows: for norfloxacin (Nor), disk content = 10, R ≤ 12, and S ≥ 17; for ofloxacin (Oflox), disk content = 5, R ≤ 12, and S ≥ 16; for ciprofloxacin (Cipro), disk content = 5, R ≤ 15, and S ≥ 21; for lomefloxacin (Lom), disk content = 10, R ≤ 18, and S ≥ 22; and for nalidixic acid (Nal), disk content = 30, R ≤ 13, and S ≥ 19.