Table 2.

Expression of chorismate synthase (CS), phenylalanine-ammonia-lyase (PAL), NADPH-dependent cinnamyl alcohol dehydrogenase (CAD), and chalcone synthase (CHS) genes in embryogenic lines from E00, Q8, and VA5 holm oak genotypes.

	Treatment	E00	Q8	VA5	Mean
CS	Control	0.005 ± 0.002	0.036 ± 0.004	0.038 ± 0.005	0.026 ± 0.011
	Infected	0.009 ± 0.002	0.056 ± 0.008	0.032 ± 0.005	0.032 ± 0.014
	Elicited	0.004 ± 0.000	0.038 ± 0.004	0.011 ± 0.002x	0.017 ± 0.010
	Elicited + Infected	0.009 ± 0.001	0.032 ± 0.003	0.030 ± 0.005	0.024 ± 0.008
PAL	Control	0.056 ± 0.018	0.103 ± 0.018	0.120 ± 0.037	0.093 ± 0.019
	Infected	0.104 ± 0.024	0.155 ± 0.025	0.116 ± 0.035	0.125 ± 0.016
	Elicited	0.044 ± 0.008	0.110 ± 0.012	0.021 ± 0.002	0.058 ± 0.027
	Elicited + Infected	0.131 ± 0.023	0.086 ± 0.015	0.135 ± 0.046	0.117 ± 0.016y
CAD	Control	0.205 ± 0.072	0.306 ± 0.090	0.450 ± 0.027	0.320 ± 0.071
	Infected	0.284 ± 0.074	0.382 ± 0.051	0.424 ± 0.019	0.363 ± 0.042
	Elicited	0.212 ± 0.026	0.398 ± 0.039	0.467 ± 0.075	0.359 ± 0.076
	Elicited + Infected	0.360 ± 0.112	0.338 ± 0.005	0.400 ± 0.154	0.366 ± 0.018
CHS	Control	0.017 ± 0.004	0.007 ± 0.002	0.024 ± 0.008	0.016 ± 0.005
	Infected	0.013 ± 0.002	0.007 ± 0.002	0.005 ± 0.001y	0.008 ± 0.002
	Elicited	0.014 ± 0.002	0.015 ± 0.003	0.015 ± 0.002	0.015 ± 0.000
	Elicited + Infected	0.012 ± 0.002	0.006 ± 0.001y	0.003 ± 0.001y	0.007 ± 0.003

Samples were taken from control and elicited with 50 μM methyl-jasmonate embryogenic lines, before and after infection with a suspension of P. cinnamomi. Data are mean ± SE of triplicated amplifications performed with three biological replicates of each treatment and line.

^xSignificantly affected by elicitation, p < 0.05 according to t-test.

^ySignificantly affected by infection, p < 0.05 according to t-test.