| Component | Amount (μl) |
|---|---|
| Sample from Step 54 | 25.5 |
| Denatured 5′ SR Adaptor for Illumina from Step 55 | 1 |
| 5′ Ligation reaction buffer (10×) | 1 |
| 5′ Ligation enzyme mix | 2.5 |
| Total | 30 |
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