Table 1.

Primers and probe used within this study.

Target	Name	Sequences 5′-3′	Amplicon size (bp)
Alphitobius diaperinus specific cadherin target	Alphi-Dia-Cad-F	CCAAGTGACTCTCATCATTCAGGAT	134
	Alphi-Dia-Cad-R	CTGAAACCGTAATGTCTAGTTCACCTA
	Alphi-Dia-Cad-P	FAM- CCATTGCAGATCCAAGTCCCCGAAA -TAMRA
A. diaperinus large cadherin target	Alphi-Cad-Seq-F	GAAGTGCCTGATCCCAGTGC	208
	Alphi-Cad-Seq-R	TGAGTTCTGCTGTGTAAAGTGCG
A. diaperinus COI targets	COI-Alphi-F	CGTAGATAAATTTACAGTTTATTGCC	760
	COI-Alphi-R	CAGGATGTCCAAAAAATCAAAATAA
	COI-Alphi-F2	CAGGATTCGGAATAATTTCTCA	758
	COI-Alphi-R2	TGCAGGAGGAGTTCTTT