Table 10.
Cq values obtained on dilutions of genomic material used for efficiency calculation and for LOD95% for the transferability test.
| Copy number of target | Cq (mean value) ± SD (σ) and (n) |
|---|---|
| 5,000 | 27.26 ± 0.11 (24) |
| 2,500 | 28.47 ± 0.16 (24) |
| 1,000 | 29.92 ± 0.11 (24) |
| 500 | 31.05 ± 0.14 (24) |
| 100 | 33.37 ± 0.23 (24) |
| 10 | 37.08 ± 0.80 (60) |
For efficiency, each concentration was analyzed in 6 replicates and on 4 PCR plates (n = 24). For LOD95%, the concentration at 10 copies was analyzed in 60 replicates on 1 PCR plate (n = 60).