Table 3.

Experimental conditions tested to evaluate the robustness of the described Alphitobius diaperinus PCR test.

PCR machine	Lightcycler 480 (Roche Diagnostics Ltd) and QuantStudio 5 (Applied Biosystems)
PCR reagent kit	Universal Mastermix (Diagenode s.a.) and ABI Taqman 2x Universal PCR Master mix (Applied Biosystems)
Annealing temperature	59 and 61°C
Primer concentration	Minus 30%	Standard	Standard	Standard	Standard
Probe concentration	Standard	Minus 30%	Standard	Standard	Standard
PCR volume	Standard (20 μL mix + 5 μL DNA)	Standard (20 μL mix + 5 μL DNA)	Standard (20 μL mix + 5 μL DNA)	Standard + 1 μL Mastermix (21 μL mix + 5 μL DNA)	Standard – 1 μL Mastermix (19 μL mix + 5 μL DNA)