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Cq values obtained on dilutions of plasmid material used for efficiency calculation and for LOD95%.
| Copy number of target | Cq (mean value) ± SD (σ) and (n) |
|---|---|
| 5,000 | 26.83 ± 0.06 (24) |
| 2,500 | 27.85 ± 0.11 (24) |
| 1,000 | 29.08 ± 0.09 (24) |
| 500 | 30.08 ± 0.11 (24) |
| 100 | 32.39 ± 0.23 (24) |
| 10 | 36.14 ± 0.50 (60) |
For efficiency, each concentration was analyzed in six replicates and on four PCR plates (n = 24). For LOD95%, the concentration at 10 copies was analyzed in 60 replicates on 1 PCR plate (n = 60).
