Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Cq values obtained on dilutions of genomic material used for efficiency calculation and for LOD95%.
| Copy number of target | Cq (mean value) ± SD (σ) and (n) |
|---|---|
| 5,000 | 27.55 ± 0.07 (24) |
| 2,500 | 28.60 ± 0.07 (24) |
| 1,000 | 29.87 ± 0.09 (24) |
| 500 | 30.89 ± 0.12 (24) |
| 100 | 33.21 ± 0.19 (24) |
| 10 | 36.78 ± 0.48 (60) |
For efficiency, each concentration was analyzed in six replicates and on four PCR plates (n = 24). For LOD95%, the concentration at 10 copies was analyzed in 60 replicates on 1 PCR plate (n = 60).
