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Effect of heat stress at different temperatures on EMSC proliferation. After 72 hours of treatment with heat stress, (a) Ki-67 expression was determined using immunofluorescence staining, and 200 cells cultured under each condition from at least 6 independent fields were counted. Ki-67-positive cells are presented as a percentage of the total population. (b) Cell proliferation was measured using the CCK-8 assay. (c) Graphs showing the percentage of Ki-67-positive cells, bar = 25 μm.
