Figure 4.

Osteogenesis of EMSCs. The expression of osteogenic genes, including OPN, OCN, COL I, and RUNX2, was increased substantially in the heat stress-treatment group compared with the control groups after osteogenic differentiation for 7 days and 14 days. (e) Increased levels of osteogenesis-associated proteins in heat stress-treated EMSCs. (f) Changes in the expression of bone-specific proteins on day 14 of differentiation, as detected by western blotting. OM = osteogenic-inducing media; NM = normal media. ^∗P < 0.05 and ^∗∗P < 0.01.