Figure 2.

Characterization of donor testis biopsies from infant boys with cryptorchidism. (A) The testis biopsies were cut into three parts. The bigger biopsy was cryopreserved in a biobank for fertility preservation, and the size of the other two pieces was similar, one for pathological assessment of the fertility potential and germ cell density, the other for experimental use. All four figures were from a testis biopsy from a 0.9-year-old infant boy with bilateral cryptorchidism (Patient ID #1). Germ cells per tubular cross section (G/T), A_dark spermatogonia per tubular cross section (AdS/T). G/T figure: four germ cells (black arrow) within one seminiferous tubule (black circle), scale bar, 50μm; AdS/T figure: an A_dark spermatogonium (black arrow), scale bar, 25 μm; total number of germ cells figure: scale bar, 50 μm; surface area figure: 1.54 mm², scale bar, 500 μm. (B) The number of germ cells (G/T) and A_dark spermatogonia (AdS/T) per tubular-cross section from 11 testis biopsies, x-axis represents age in years. (C) Germ cell density was the total number of germ cells per mm³ on tubular sections from 11 testis biopsies, x-axis represents age in years. Dots represent the mean value; error bars represent standard deviation (SD).