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. 2022 Mar 10;16:808598. doi: 10.3389/fncel.2022.808598

FIGURE 1.

FIGURE 1

LG dual reporter assays to measure HEXB promoter activity. (A) Diagram for LG dual reporter assay driven by HEXB promoters derived from mouse, human, or monkey cells. (B) HEK293T cells were transfected with mouse 330 bp Hexb promoter-driven dual LG reporter (mHexb-330) and no Hexb 5’UTR-driven dual LG reporter (mHexB-no UTR, −330 to −97) plasmid in 96-well plate, where Empty promoterless LG reporter vector served as negative control. At 48–72 h after transfection, gdLuc levels in culture media were measured to determine the Hexb promoter activities. (C) Representative images were taken to show GFP expression in HEK293T cells 72 h after transfection (scale bar, 100 μm). ***p < 0.001 analyzed with student’s t-test compared with empty vector transfection control (n = 4).