Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 10;16:808598. doi: 10.3389/fncel.2022.808598

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Shah, Wong, Ellis, Bodnar, Saribas, Ting, Wei, Tang, Wang, Wang, Ling, Margolis, Garcia, Hu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Activity of microglia-related promoters, including HEXB and CD68, in the induction of gene transcription in HEK293T cells. (A) Different sizes of mouse, human, or monkey HEXB promoter-driven dual-LG reporters were transfected into HEK293T cells in 96-well plate. At 72 h after transfection, gdLuc levels in culture media were measured to determine the HEXB promoter activity. Cells transfected with plasmids driven by other microglia-related promoters, such as CD68, were also included, and transcription activity were determined by measuring luciferase levels in culture supernatants. (B) Representative images after transfection are shown (scale bar, 100 μm). ****p < 0.0001, compared with empty vector transfection controls (One-way ANOVA, n = 4). #p < 0.05; ##p < 0.01; ###p < 0.001, compared with indicated groups (t-test, n = 4). m, mouse; h, human; mk, monkey.