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. 2022 Mar 10;16:808598. doi: 10.3389/fncel.2022.808598

FIGURE 6.

FIGURE 6

Human HEXB promoter-induced gene transcription in comparison to other microglia gene promoters in primary human microglia and astrocytes. Microglia-specific promoter-driven dual-LG reporters were transfected into human primary microglia (A,C) or human primary astrocytes (E,G) in 96-well plate. After transfection, GFP+ cells were observed with microscopy (A,E), and gdLuc levels (C,G) in culture media were measured to determine the HEXB promoter activities. Similarly, promoter-driven dual-LG reporter were transfected into primary rhesus macaque microglia (B,D) or primary rhesus macaque astrocytes (F,H) in 96-well plate. GFP+ cells were observed with microscopy (B,F), and gdLuc levels in culture media (D,H) were measured to determine the HEXB promoter activities. Scale bar, 400 μm. **p < 0.01; ***p < 0.001; ****p < 0.0001; compared with empty vector transfection controls (One-way ANOVA, n = 3). #p < 0.05; ##p < 0.01 (t-test, n = 3).