Table 2.

Virucidal efficacy of laundry sanitizers tested per EN 14,476:2013 + A2:2019 on SARS-CoV-2 and HCoV 229E in suspension studies in the presence of an organic load.

Active ingredient and tested concentration	Temperature	Contact time (minutes)	Log10 reduction in infectious titer achieveda
			Alpha-coronavirus	Beta-coronavirus
			HCoV 229E	SARS-CoV-2
PCMX (0.033% in 100 ppm AOAC hard water), 1:50 dilution of the product in hard water	20 ± 1 °C	15	≥ 5.2b	≥ 5.2b
QACd (0.055% in hard water), 1:42 dilution of the product	20 ± 1 °C	15	Not tested	≥ 5.0c
QACd (0.057% in hard water), 1:42 dilution of the product	20 ± 1 °C	15	Not tested	≥ 4.2b

HCoV 229E human coronavirus 229E, PCMX p-chloro-m-xylenol, QAC quaternary ammonium compound, SARS-CoV-2 severe acute respiratory syndrome coronavirus 2, w/v weight to volume, w/w weight to weight.

^aIn all cases, a single replicate measurement was used to generate the data point.

^bNeutralizer used: minimal essential medium + 10% fetal bovine serum; organic load: 0.3% bovine serum albumin + 0.3% erythrocyte solution.

^cCytotoxicity was reduced by passage of the virus/test substance through a Sephadex LH-20 gel filtration column; organic load: 0.3% bovine serum albumin.

^dQAC included benzalkonium chloride and dialkyldimethylammonium chloride.