Table 3.

Virucidal efficacy of a laundry sanitizer tested per ASTM E1053-20 against coronaviruses and influenza viruses dried on a glass surface in the presence of a 5% fetal bovine serum organic load.

Active ingredient and tested concentration	Temperature (% RH)	Contact time (minutes)	Log10 reduction in infectious titer achieveda
			2009-H1N1 Influenza A virus	Influenza B virus	Alpha-coronavirus	Beta-coronavirus
					HCoV 229E	SARS-CoV-2
QACd (0.08%) 1:28 of product in 400 ppm AOAC hard water	20 ± 1 °C (25–40%)	5	≥ 5.0, ≥ 5.0b	≥ 3.0, ≥ 3.0b	≥ 4.5, ≥ 4.5b	≥ 3.0, ≥ 3.0, ≥ 3.0c

AOAC Association of Official Analytical Chemists, HCoV 229E human coronavirus 229E, QAC quaternary ammonium compound, SARS-CoV-2 severe acute respiratory syndrome coronavirus 2, RH relative humidity.

^aWhere multiple values are displayed, this reflects the testing of multiple independent lots of the test sanitizer. In all cases, a single replicate measurement was used to generate the data point.

^bCytotoxicity was reduced by passage of the virus/test substance through a Sephadex LH-20 gel filtration column.

^cNeutralizer used: minimal essential medium + 10% fetal bovine serum + 0.5% polysorbate 80 + 0.5% lecithin.

^d BTC 8358 + Bardac 2080.