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. 2022 Mar 10;10:815205. doi: 10.3389/fcell.2022.815205

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Copyright © 2022 Kim, Jang, Kim, Jang, Cho, Han, Song, Kim and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of lyophilized Milk-exo and conservation of in vivo hair growth ability. (A) Western blot analysis of exosome markers (TSG101, Alix) and milk specific proteins (MFG-E8 and lactoferrin). (B) Representative TEM image and size distribution diagram of lyophilized Milk-exo. (C) Representative images of hair regeneration after intradermal injection of saline, Milk-exo and FD Milk-exo. (D) Quantification of hair coverage level (n = 3, ****p < 0.0001 versus saline, two-way ANOVA, Dunnett’s multiple comparisons test). (E) Mean visual score measurements (n = 3; ****p < 0.0001, two-way ANOVA, Dunnett’s multiple comparisons test).