Figure 3.

Average spectra of LD from (A) MCF-10A and (B) MDA-MB-231 cells under various methionine and insulin concentrations. Manual identification of potential Raman peak targets is highlighted with vertical lines and labels. For example, the CD_Lipid peak in the cell silent region at 2135cm^-1 has a noticeable increase relative to the CD_Protein peak at 2180cm^-1 in the excess methionine groups. This could indicate preference for de novo lipogenesis in excess methionine environments. (C) Raman spectra of common lipid species in LD, in descending order of prevalence.