Figure 5.

Quantitative de novo lipid synthesis (A) Normalized CD_L intensities show excess methionine stimulates de novo lipogenesis. (B, C) CD_L Ratios show violin box-plots of de novo lipid synthesis CD_L ratios for MCF10A and MDA-MB-231, respectively. CDL : CH2 illustrates the relative de novo lipid synthesized to total ascribable lipid content. Balanced 2-way ANOVA with constrained sum of squares results of CD_L ratios shows methionine concentration significantly influenced the CD_L: CD_P ratio in both MCF10A and MDA-MB-231 lipid droplet spectra with rejection levels of ^#P < 0.05 and ^####P < 0.0001, respectively. (D, E) CD_L: CD_P illustrates the relative de novo lipid and protein synthesized biomolecules for MCF10A and MDA-MB-231, respectively. Values were taken from spectra of lipid droplets only. while. There was no significant evidence of interactions between these two independent variables for these ratios. Balanced 2-way ANOVA with constrained sum of squares results of CD_L ratios indicate insulin significantly influenced the CD_L: CH₂ ratio in MCF-10A lipid droplet spectra with a rejection level of ^####P < 0.0001, but no significant evidence of interactions between these two independent variables. However, in TNBC insulin, methionine, and the interaction term significantly influenced the CD_L : CH₂ ratio in MDA-MB-231 lipid droplet spectra with a rejection level of ^####P < 0.0001 and ^#P < 0.05 for the individual and interaction terms, respectively.