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. Author manuscript; available in PMC: 2022 Mar 29.
Published in final edited form as: Neurosci Res. 2021 Oct 5;176:73–78. doi: 10.1016/j.neures.2021.10.001

Fig. 3. Cdkl5 knockdown neurons display no change in tubulin expression and post-translational modification.

Fig. 3.

(A) Representative western blot of ctrl-sh and Cdkl5-sh lentiviral vectors in a time course experiment at DIV5, 7, 13 and 15. Quantification of γTubulin (n = 3, in (B), Tuj1 (n = 4, in C), Acetylated tubulin (n = 4, in D), and Tyrosinated tubulin (E n = 3), Data are average fold changes of ctrl-sh neurons. GAPDH was used as loading control for protein level normalization. Quantifications are relative to ctrl-sh neurons (unpaired Student’s ns = not significant, error bars indicate ± SEM).