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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Control Release. 2022 Jan 22;343:267–276. doi: 10.1016/j.jconrel.2022.01.024

Figure 4: CpG complexed with ELP-K12 shows greater cellular uptake and immune stimulation than CpG alone.

Figure 4:

(A) Production of NO by Raw264.7 cells induced by ELP-K12/CpG-ODN complex at multiple N:P ratios as measured by a colorimetric Griess assay. (B) Flow cytometry analysis of cellular uptake of fluorescently labeled CpG by RAW264.7 cells treated with ELP-K12/CpG or CpG alone. Confocal fluorescence microscopy of cellular uptake of FITC-labeled CpG (green) alone (C) and with AlexaFluor350-labeled ELP-K12 (blue) (D). Secondary labeling with AlexaFluor594 marks LAMP1, designating the early endosome (red). *p<0.05 (ANOVA, Tukey), **p<0.05 (student t-test)