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. 2022 Mar 10;9:835302. doi: 10.3389/fmolb.2022.835302

TABLE 1.

Summary of affinities (KD), association (kON) and dissociation constants (kOFF) and the coefficient of determination (R2) determined for Miro1-Nbs by BLI.

Nanobody K D (nM) kON (105 M−1 s−1) kOFF (10−4s−1) R2
M11 3.34 2.55 ± 0.02 8.54 ± 0.03 0.97
M25 29.50 1.36 ± 0.04 0.40 ± 0.28 0.80
M41 1.96 3.01 ± 0.01 5.89 ± 0.02 0.99
M85 7.75 1.00 ± 0.01 7.79 ± 0.02 0.99
M114 4.61 0.88 ± 0.01 4.04 ± 0.01 0.99
M119 2.80 2.25 ± 0.01 6.31 ± 0.02 0.99
M189 2.99 1.70 ± 0.01 5.07 ± 0.02 0.98