Figure 5.
Role of ERα in G-induced intracellular expression of hsa-miR-500a-5p. PBMC (106/mL) were treated with ICI 182,780 (1 µM) for 15 minutes, and subsequently with G (0.1 µg/mL) for 72 h. Total RNA was extracted, retro-transcribed, and hsa-miR-500a-5p detected through Real-Time PCR. Fold-changes (2−ΔΔCt) are reported, using hsa-RNU6-2 as negative control. Each dot represents the fold change of hsa-miR-500a-5p expression induced by G over control for each individual, whereas rhombuses represent the fold change of hsa-miR-500a-5p expression obtained from cells treated with ICI 182,780 and G over ICI-treated cells. Each value represents the mean ± SEM, n = 5 donors. Statistical analysis was performed with Paired t test, with *p < 0.05 vs G-treated cells.