Fig. 1.

RSV activates the expression of RNase2 in THP1-induced macrophages. 10⁶ THP-1 cells/well were seeded in 6-well tissue culture plate and induced by 50 nM of PMA treatment. After induction, macrophages were exposed to RSV under MOI = 1 for 2 h and then cells were washed and replaced with fresh RPMI + 10%FBS (0 h time point post of inoculation). At each time point post of inoculation, the supernatant and cells were collected to quantify the expression of RNase2. A qPCR detection of relative expression of RNase2 gene; B concentration of the cells was controlled as 10⁶ cells/mL, secreted RNase2 in culture supernatant was measured by ELISA and normalized with alive cell number detected by MTT assay; C intracellular RNase2 protein in macrophage was detected by WB; “ + ” and “ − ” indicate with or without RSV, respectively; * and ** indicate the significance of p < 0.05 and p < 0.01, respectively