Table 2.
Comparison of RNase2 cleavage specificity on ncRNA in THP1-derived macrophages (this study) with synthetic RNA substrates in vitro
| Synthetic RNA | Base preference | Ref | Cellular RNA* | Base preference |
|---|---|---|---|---|
| B1 | ||||
| polyU/polyC | U/C ~ 2 | [98] | tRNA (loops) | U/C ~ 1.2 |
| polyU/polyC | U/C ~ 1 | [97] | tRNA (stems) | |
| ORNs# | U/C ~ 1.3 | [28] | miRNA |
U–U (loops) G–G (stems) |
| B2 | ||||
| UpA/UpG | (A/G ~ 47) | [97] | tRNA (loops) | A |
| UpA/UpG | (A/G > 100) | [59] | miRNA | |
| ORNs# | G ≥ A ≥ U ≥ C | [28] |
#ORNs (short single-stranded oligonucleotides: ssRNA40, 9.2 s RNA, and R2152)
*This study