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. 2022 Mar 3;27(3):315–328. doi: 10.1007/s00775-022-01935-6

Table 2.

Excitation (λEX) and emission maxima (λEM) of InQ3 and GaQ3 complexes and fluorescence lifetime (τi) and amplitude (αi) values alone and in the presence of HSA in various concentrations at pH = 7.40. {ccomplex = 10 μM; cHSA = 2–10 μM; T = 25.0 °C; in 10 mM HEPES, 25 mM NaHCO3, 100 mM NaCl buffer}a

InQ3b GaQ3
λEX max (nm) 254, 370 367c
λEM max (nm) 540 532 c
Fluorescence lifetime parameters of the complexes alone
 τ1 (ns) 0.80 ± 0.03 0.54 ± 0.04
 τ2 (ns) 1.41 ± 0.07 1.23 ± 0.03
 α1 (%) 75 ± 4 57 ± 5
 α2 (%) 25 ± 4 43 ± 5
Two additional lifetime components in the presence of HSA
 τ3 (ns) 5.7 ± 0.3 5.0 ± 0.3
 τ4 (ns) 13.8 ± 0.6 14.0 ± 0.2

aValues are the average of at least three measurements. bData for InD3: λEX max = 295 nm, λEX max = 354 nm. cData taken form Refs. [22, 29]