Table 2.
Excitation (λEX) and emission maxima (λEM) of InQ3 and GaQ3 complexes and fluorescence lifetime (τi) and amplitude (αi) values alone and in the presence of HSA in various concentrations at pH = 7.40. {ccomplex = 10 μM; cHSA = 2–10 μM; T = 25.0 °C; in 10 mM HEPES, 25 mM NaHCO3, 100 mM NaCl buffer}a
| InQ3b | GaQ3 | |
|---|---|---|
| λEX max (nm) | 254, 370 | 367c |
| λEM max (nm) | 540 | 532 c |
| Fluorescence lifetime parameters of the complexes alone | ||
| τ1 (ns) | 0.80 ± 0.03 | 0.54 ± 0.04 |
| τ2 (ns) | 1.41 ± 0.07 | 1.23 ± 0.03 |
| α1 (%) | 75 ± 4 | 57 ± 5 |
| α2 (%) | 25 ± 4 | 43 ± 5 |
| Two additional lifetime components in the presence of HSA | ||
| τ3 (ns) | 5.7 ± 0.3 | 5.0 ± 0.3 |
| τ4 (ns) | 13.8 ± 0.6 | 14.0 ± 0.2 |