Fig. 2.

Equilibriums of cytosolic helically folded and unfolded physiological αS is disturbed in PD and DLB patients. a Representative Western blot of αS^H and αS^U in controls, DLB and PD patients. Each piece of brain was analyzed in duplicates (frontal cortex FC1 and FC2). The cross-linking reaction was performed in technical triplicates alongside one non-cross-linked (PBS) sample. The Western blot demonstrates reduced αS^H to αS^U ratios in DLB and PD patients compared to the control. DSG “+” = cross-linked sample. DSG “−” = non-cross-linked sample. Green = αS, red = DJ1. b Significant reduction of αS^H/αS^U ratio in the frontal cortex comparing controls (n = 19) with DLB (n = 14) and PD (n = 8) patients. c Significant reduction of αS^H/αS^U ratio in the cingulate cortex comparing controls (n = 7) to DLB (n = 7) patients. No significant alteration of the αS^H/αS^U equilibriums in the cingulate cortex comparing controls (n = 7) and PD (n = 6) patients. d Significant difference of decreased αS^H/αS^U ratios and increased Braak LB staging of the frontal cortex (p < 0.001). e Significant difference of decreased αS^H/αS^U ratios and increased Braak LB staging of the cingulate cortex (p = 0.02)