TABLE 2.
PCR primers used in the identification of integrons and resistance genes
| Gene | PCR primer sequences 5′-3′ | Expected size (bp) | GenBank accession no. | Reference strain (plasmid) | Source (reference) |
|---|---|---|---|---|---|
| Integron | 5′-CS, GGC ATC CAA GCA GCA AG; 3′-CS, AAG CAG ACT TGA CCT GA | ?a | M73819 | NAb | P. Roy (11) |
| pse1 | L, AAT GGC AAT CAG CGC TTC CC; R, GGG GCT TGA TGC TCA CTC CA | 586 | Z18955 | Salmonella serovar Newport E1572 | G. Jacoby (10) |
| aadA2 | L, TGT TGG TTA CTG TGG CCG TA; R, GCT GCG AGT TCC ATA GCT TC | 381 | X68227 | Escherichia coli(pSa) | D. Taylor (3) |
| sulI | qacEΔ1, TAG TTG GCG AAG TAA TCG CA; orf5, AGC TTG TGC AGA TAT GCG G; sulI, TGA AGG TTC GAC AGC AC | 1,581 | U49101 | Proteus mirabilis 88071820 | P. Roy (11) |
| aphA-1 | L, TTA TGC CTC TTC CGA CCA TC; R, GAG AAA ACT CAC CGA GGC AG | 489 | U63147 | Escherichia coli JE2571(pHH1457) | D. Taylor (4) |
| intI1 | L, GCC TTG CTG TTC TTC TAC GG; R, GAT GCC TGC TTG TTC TAC GG | 558 | X12870 | NA | P. Roy (11) |
| pasppflo-like | L, CAC GTT GAG CCT CTA TAT GG; R, ATG CAG AAG TAG AAC GCG AC | 868 | AFO71555 | NA | GenBank (5) |
| tet(A) | L, GCT ACA TCC TGC TTG CCT TC; R, CAT AGA TCG CCG TGA AGA GG | 210 | X61367 | Escherichia coli D20-15(pSL18) | S. Levy (14) |
| tet(B) | L, TTG GTT AGG GGC AAG TTT TG; R, GTA ATG GGC CAA TAA CAC CG | 659 | J01830 | Escherichia coli D20-16(pRT11) | S. Levy (13) |
| tet(C) | L, CTT GAG AGC CTT CAA CCC AG; R, ATG GTC GTC ATC TAC CTG CC | 418 | J01749 | Escherichia coli D20-6(pBR322) | S. Levy (13) |
| tet(D) | L, AAA CCA TTA CGG CAT TCT GC; R, GAC CGG ATA CAC CAT CCA TC | 787 | L06798 | Escherichia coli D22-2(pSL106) | S. Levy (13) |
| tet(E) | L, AAA CCA CAT CCT CCA TAC GC; R, AAA TAG GCC ACA ACC GTC AG | 278 | L06940 | Escherichia coli HB101(pSL1504) | S. Levy (12) |
| tet(G) | L, GCT CGG TGG TAT CTC TGC TC; R, AGC AAC AGA ATC GGG AAC AC | 468 | S52437 | Escherichia coli(pJA8122) | T. Aoki (24) |
| tet(G) | L, CAG CTT TCG GAT TCT TAC GG; R, GAT TGG TGA GGC TCG TTA GC | 844 | S52437 | Escherichia coli(pJA8122) | T. Aoki (24) |
| tet(S) | L, CAT AGA CAA GCC GTT GAC C; R, ATG TTT TTG GAA CGC CAG AG | 667 | X92946 | Escherichia coli(pAT451) | M. Roberts (6) |
a
?, variable size depending on the inserted gene(s).
b
NA, not available.