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. 2022 Mar 10;12:798401. doi: 10.3389/fonc.2022.798401

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Copyright © 2022 Cui, Liu, Wu, Liang, Dai, Zhang and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical staining to evaluate the association between del-AHNAK2 ^mut and activated immune microenvironment. (A) Computed tomography (CT) imaging of the representative MPLC patient. (B–D) Quantitative immunohistochemistry (IHC) analysis of CD3 (B), GZMB (C), and IFNG (D) protein expression in the del-AHNAK2 ^mut and AHNAK2 ^wt groups (n = 5/group). Representative micrographs of these samples are shown. Data represent the mean ± SD. (Mann–Whitney U test; *p < 0.05, **p < 0.01).