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. 2022 Mar 28;8:134. doi: 10.1038/s41420-022-00945-2

Fig. 5. Blocking AT1R prevents the electrophysiological abnormalities and cardiac oxidative stress in hiPSC-CMs models of ACM.

Fig. 5

A Representative immunofluorescence images of JC-1 labeling showed a reduction in green fluorescence intensity after losartan administration compared with that after alcohol treatment (n = 20). Scale bar = 25 μm. B Representative immunofluorescence images of CM-H2DCFDA showed that losartan restored the level of mitochondrial oxidative stress induced by 100-mM alcohol. (n = 10) C Representative immunofluorescence images of Texas red labeling showed that losartan restored the level of the oxidative stress induced by 100-mM alcohol. (n = 10) D Western blotting and quantitative statistics showed that losartan restored the protein expression level of NOX4 induced by 100-mM alcohol (n = 5). E–G RTCA for dynamically monitoring cell index waveform, amplitude and beat frequency showed that losartan restored the electrophysiological disturbance of hiPSC-CMs induced by 100 mM alcohol [F (n = 39); G (n = 41)]. Results are presented as means ± standard error of the mean (**P < 0.01; two-sided Student’s t-test or one-way analysis of variance).