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. 2022 Mar 28;5:275. doi: 10.1038/s42003-022-03226-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a An overlay of chain B from the structures of the reduced (light gray) and oxidized (teal) states of RexT. A box is included to highlight the Cys residues involved in disulfide bond formation. The formation of the disulfide bond requires Cys41 to undergo a large conformational change. b Comparison of the eight-membered vicinal disulfide bond geometry in RexT and 1, 5-alpha-L-arabinanase³⁷ (PDB ID: 3CU9). The ω angles of both RexT and 1, 5-alpha-L-arabinanase show a large deviation from the ideal values. c Upon disulfide bond formation in RexT, the movement of Cys41 causes disruptions in the interactions between the thiol-group and the sidechain of Asn77 as well as interactions between the Cys41 backbone amide and the backbone of His75. Both Asn77 and His75 are found in the wing portion of the wHTH motif involved in DNA binding. d The B factors of the reduced (top panel, RexT_red) and oxidized (bottom panel, RexT_ox) states of RexT are illustrated in a continuum from blue, white to red. Blue corresponds to a lower B factor and red represents a higher B factor. The reduced structure is colored on a scale from 10 to 30, whereas the oxidized structure is colored on a scale from 20 to 60.