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. 2022 Mar 28;5:275. doi: 10.1038/s42003-022-03226-x

Fig. 7. A mechanistic proposal for H2O2-mediated disulfide bond formation in RexT.

Fig. 7

Based on the crystal structures of oxidized and reduced RexT, as well as the H2O2 consumption assays performed in this work, we propose that a disulfide bond is formed between Cys40 and Cys41. In addition, the first step of disulfide bond formation, or the conversion of the Cys41 sidechain into a sulfenic acid moiety requires a tetrad of residues, Cys41, Arg61, Thr68, and Asn77.