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. 2022 Mar 28;13:1637. doi: 10.1038/s41467-022-29183-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The experimental outline. 196 lines of the Drosophila melanogaster genetic reference panel (DGRP) were raised on six different diets: high-protein (HPD), high-sugar (HSD), high-fat-coconut-oil (HFDcoco), high-fat-lard (HFDlard), Western (WD), and high-starch (HStD). First-instar larvae were collected onto the diets in a controlled density of 30 larvae per vial, and at four replicate vials per diet. The experiment was run in cohorts of 10 strains (all diets at the same time). Pupation was monitored daily to establish pupation kinetics of each strain across the diets and finally total eclosion was scored two weeks after. HPD was composed of 10% (w/v) dry baker’s yeast, and the other diets had the same amount of yeast as a base. HSD was supplemented with 20% (w/v) sucrose, HFDcoco with 20% (w/v) coconut oil, HFDlard with 20% (w/v) lard, WD with 10% (w/v) sucrose and 10% (w/v) lard, and HStD with 20% (w/v) potato starch. b Survival of the 196 DGRP strains to pupal stage (pupation) and to adult (eclosion) across the six diets. Pupation rate standard deviations (among lines) of 0.194 (HSD), 0.188 (HFDcoco), 0.162 (WD), 0.145 (HPD), 0.144 (HFDlard), and 0.140 (HStD). Eclosion rate standard deviations (among lines) of 0.211 (HSD), 0.203 (HFDcoco), 0.166 (HPD), 0.138 (HStD), 0.099 (HFDlard), and 0.092 (WD). c HPD-normalized survival of the 196 DGRP strains to pupal stage (pupation) and to adult (eclosion) across the other five diets. Source data are provided as a Source Data file.